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UBR4 antibody (C-Term)

This anti-UBR4 antibody is a Goat Polyclonal antibody detecting UBR4 in IHC and ELISA. Suitable for Human.
Catalog No. ABIN570777

Quick Overview for UBR4 antibody (C-Term) (ABIN570777)

Target

See all UBR4 Antibodies
UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Reactivity

  • 24
  • 13
  • 11
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 19
  • 5
Goat

Clonality

  • 20
  • 4
Polyclonal

Conjugate

  • 17
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This UBR4 antibody is un-conjugated

Application

  • 13
  • 10
  • 9
  • 5
  • 5
  • 3
  • 3
  • 3
  • 1
  • 1
Immunohistochemistry (IHC), ELISA

Grade

Verified
  • Binding Specificity

    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    C-Term

    Purpose

    UBR4 / ZUBR1

    Sequence

    NTTRATSREE KNLQ

    Cross-Reactivity

    Cow, Dog, Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10 μg/mL.

    Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574 kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody i

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

    Alternative Name

    UBR4

    Background

    UBR4, ubiquitin protein ligase E3 component n-recognin 4, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600, retinoblastoma-associated factor 600-like protein, zinc finger, UBR1 type 1

    Gene ID

    23352, 69116, 313658

    NCBI Accession

    NP_065816
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