UBR4 antibody (C-Term)
Quick Overview for UBR4 antibody (C-Term) (ABIN570777)
Target
See all UBR4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- C-Term
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Purpose
- UBR4 / ZUBR1
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Sequence
- NTTRATSREE KNLQ
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Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.
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Isotype
- IgG
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Application Notes
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Immunohistochemistry: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10 μg/mL.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574 kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody i
Peptide ELISA: antibody detection limit dilution 1:128000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))
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Alternative Name
- UBR4
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Background
- UBR4, ubiquitin protein ligase E3 component n-recognin 4, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600, retinoblastoma-associated factor 600-like protein, zinc finger, UBR1 type 1
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Gene ID
- 23352, 69116, 313658
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NCBI Accession
- NP_065816
Target
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