beta-2 Microglobulin antibody

Catalog No. ABIN5707815

The Rabbit Monoclonal anti-beta-2 Microglobulin antibody has been validated for IHC (p). It is suitable to detect beta-2 Microglobulin in samples from Human and Non-Human Primate.

Quick Overview for beta-2 Microglobulin antibody (ABIN5707815)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Non-Human Primate

Host: Rabbit

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B2M-1857R

Product Details anti-beta-2 Microglobulin Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant B2M antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant B2M antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant B2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: Beta-2 Microglobulin

Background: Recognizes a protein of 12  kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process