Recombinant HCG beta antibody

Catalog No. ABIN5707874

This Rabbit Monoclonal antibody specifically detects HCG beta in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant HCG beta antibody (ABIN5707874)

Target: HCG beta

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HCG beta antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: HCGb-1996R

Product Details anti-HCG beta Antibody

Purpose: Recombinant HCG-beta Antibody / Rabbit Monoclonal (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for this recombinant HCG-beta antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant HCG-beta antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant HCG-beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for HCG beta

Target: HCG beta

Alternative Name: HCG-beta

Background: This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.