SOX10 antibody (AA 115-269)

Catalog No. ABIN5707895

This Mouse Monoclonal antibody specifically detects SOX10 in IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for SOX10 antibody (AA 115-269) (ABIN5707895)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RSOX10-1074

Product Details anti-SOX10 Antibody

Binding Specificity: AA 115-269

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Amino acids 115-269 from the human protein were used as the immunogen for the recombinant SOX10 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the recombinant SOX10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. WB: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant SOX10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: This mAb recognizes a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX10 is moderately to strongly expressed in desmoplastic melanomas. SOX10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX10 expression is also observed in mast cells.

Pathways: Chromatin Binding