Nkx2-2 antibody (AA 1-119)

Catalog No. ABIN5707913

This Rabbit Monoclonal antibody specifically detects Nkx2-2 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Nkx2-2 antibody (AA 1-119) (ABIN5707913)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NX2-2198R

Product Details anti-Nkx2-2 Antibody

Binding Specificity: AA 1-119

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A human partial recombinant protein (within amino acids 1-119) was used as the immunogen for this recombinant NKX2.2 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Gene ID: 4821

Pathways: Dopaminergic Neurogenesis