TYR antibody

Catalog No. ABIN5708030

This Rabbit Monoclonal antibody specifically detects TYR in IHC (p). It exhibits reactivity toward Human.

Quick Overview for TYR antibody (ABIN5708030)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TYR-2024R

Product Details anti-TYR Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for this recombinant Tyrosinase antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Tyrosinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background: Recognizes a cluster of proteins between 70-80  kDa, identified as tyrosinase. Occasionally a minor band at 55  kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.