MDFI antibody (N-Term)

Catalog No. ABIN570850

This Goat Polyclonal antibody specifically detects MDFI in ELISA. It exhibits reactivity toward Human.

Quick Overview for MDFI antibody (N-Term) (ABIN570850)

Target: MDFI (MyoD Family Inhibitor (MDFI))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MDFI antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-MDFI Antibody

Binding Specificity: N-Term

Purpose: MDFI

Sequence: YQVSGQRPSG CD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YQVSGQRPSGCD, from the N Terminus of the protein sequence according to NP_005577.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 20 kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI

Background: MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000196414, inhibitor of MyoD family a

Gene ID: 4188

NCBI Accession: NP_005577

Pathways: Maintenance of Protein Location