MDFI antibody (Internal Region)

Catalog No. ABIN570760

Product Details anti-MDFI Antibody

Target: MDFI (MyoD Family Inhibitor (MDFI))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MDFI antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: MDFI

Sequence: SKSTTSQIPL QAQED

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SKSTTSQIPLQAQED, from the internal region of the protein sequence according to NP_005577.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung (Bronchus). Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx. 70 kDa band in lysates of cell line HepG2 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI (MDFI Products)

Synonyms: MDFI antibody, I-MF antibody, I-mfa antibody, I-mf antibody, RGD1560271 antibody, MyoD family inhibitor antibody, MDFI antibody, Mdfi antibody

Background: MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000016375, OTTHUMP00000196414, inhibitor of MyoD family a

Gene ID: 4188

NCBI Accession: NP_005577

Pathways: Maintenance of Protein Location