POU5F1B antibody (Internal Region)

Catalog No. ABIN570857

The Goat Polyclonal anti-POU5F1B antibody has been validated for ELISA. It is suitable to detect POU5F1B in samples from Human.

Quick Overview for POU5F1B antibody (Internal Region) (ABIN570857)

Target: POU5F1B (POU Class 5 Homeobox 1B (POU5F1B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POU5F1B antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-POU5F1B Antibody

Binding Specificity: Internal Region

Purpose: POU5F1B

Sequence: TPGYGSPHFT ALYS

Specificity: This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPGYGSPHFTALYS, from the internal region (near C Terminus) of the protein sequence according to NP_001153014.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6 kDa

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POU5F1B

Target: POU5F1B (POU Class 5 Homeobox 1B (POU5F1B))

Alternative Name: POU5F1B

Background: POU5F1B, POU class 5 homeobox 1B, OCT4-pg1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain, class 5, transcription factor 1 pseudogene 1, octamer b

Gene ID: 5462, 5460

NCBI Accession: NP_001153014