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POU5F1B antibody (Internal Region)

Recent POU5F1B Reactivity: Human ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN570857
$253.44
Plus shipping costs $45.00
100 μg
Shipping to: United States
Delivery in 6 to 7 Business Days
  • Target See all POU5F1B Antibodies
    POU5F1B (POU Class 5 Homeobox 1B (POU5F1B))
    Binding Specificity
    • 16
    • 10
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    Human
    Host
    • 26
    • 6
    Goat
    Clonality
    • 32
    Polyclonal
    Conjugate
    • 11
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    This POU5F1B antibody is un-conjugated
    Application
    • 24
    • 23
    • 17
    • 2
    • 2
    • 1
    ELISA
    Purpose
    POU5F1B
    Sequence
    TPGYGSPHFT ALYS
    Specificity
    This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).
    Predicted Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Recent
    Immunogen
    Peptide with sequence C-TPGYGSPHFTALYS, from the internal region (near C Terminus) of the protein sequence according to NP_001153014.1.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6 kDa according to NP_001153014.1. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    POU5F1B (POU Class 5 Homeobox 1B (POU5F1B))
    Alternative Name
    POU5F1B (POU5F1B Products)
    Synonyms
    OCT4-PG1, OTF3C, OTF3P1, POU5F1P1, POU5F1P4, POU5FLC20, POU5FLC8, POU class 5 homeobox 1B, POU5F1B
    Background
    POU5F1B, POU class 5 homeobox 1B, OCT4-pg1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain, class 5, transcription factor 1 pseudogene 1, octamer binding protein 3_like sequence
    Gene ID
    5462, 5460
    NCBI Accession
    NP_001153014
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