NIPBL antibody (AA 889-902)

Catalog No. ABIN570861

This anti-NIPBL antibody is a Goat Polyclonal antibody detecting NIPBL in ELISA, IHC and IF. Suitable for Human.

Quick Overview for NIPBL antibody (AA 889-902) (ABIN570861)

Target: NIPBL (Nipped-B like Protein (NIPBL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NIPBL antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-NIPBL Antibody

Binding Specificity: AA 889-902

Purpose: NIPBL (aa889-902)

Sequence: RPDSPRVKQG DSNK

Specificity: This antibody is expected to recognize both reported isoforms (NP_597677.2, NP_056199.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPDSPRVKQGDSNK, from the internal region of the protein sequence according to NP_597677.2, NP_056199.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NIPBL

Target: NIPBL (Nipped-B like Protein (NIPBL))

Alternative Name: NIPBL

Background: NIPBL, Nipped-B homolog (Drosophila), CDLS, CDLS1, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3, IDN3-B, Scc2, SCC2 homolog, delangin, nipped-B-like, sister chromatid cohesion 2 homolog

Gene ID: 25836, 71175, 294787

NCBI Accession: NP_597677, NP_056199

Pathways: Sensory Perception of Sound, Stem Cell Maintenance