LIMS2 antibody (Internal Region)

Catalog No. ABIN570889

This Goat Polyclonal antibody specifically detects LIMS2 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for LIMS2 antibody (Internal Region) (ABIN570889)

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIMS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-LIMS2 Antibody

Binding Specificity: Internal Region

Purpose: LIMS2

Sequence: RWSNMSDALA

Specificity: This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RWSNMSDALA

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in Human Heart and Mouse Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIMS2

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Alternative Name: LIMS2

Background: LIMS2, LIM and senescent cell antigen-like domains 2, FLJ10044, PINCH2, ILK-binding protein, OTTHUMP00000203848, OTTHUMP00000203850, OTTHUMP00000203866

Gene ID: 55679

NCBI Accession: NP_001129509, NP_001154875

Pathways: Cell-Cell Junction Organization