CD20 antibody (C-Term)

Catalog No. ABIN570905

The Goat Polyclonal anti-CD20 antibody has been validated for WB, IHC and ELISA. It is suitable to detect CD20 in samples from Human.

Quick Overview for CD20 antibody (C-Term) (ABIN570905)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CD20 Antibody

Binding Specificity: C-Term

Purpose: CD20 / MS4A1 (C Terminus)

Sequence: CQDQESSPIE NDSSP

Specificity: Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CQDQESSPIENDSSP, from the C Terminus of the protein sequence according to NP_068769.2, NP_690605.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 6-7 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of Human Lymph Node and of cell line Daudi (calculated MW of 33.1 kDa according to NP_068769.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: MS4A1

Background: MS4A1, membrane-spanning 4-domains, subfamily A, member 1, B1, S7, BP35, CD20, LEU-16, MGC3969, CD20 antigen, CD20 receptor, B-lymphocyte cell-surface antigen B1

Gene ID: 931

NCBI Accession: NP_068769, NP_690605