TJP1 antibody (Internal Region)

Catalog No. ABIN570961

This Goat Polyclonal antibody specifically detects TJP1 in ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for TJP1 antibody (Internal Region) (ABIN570961)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TJP1 Antibody

Binding Specificity: Internal Region

Purpose: ZO-1

Sequence: PKPTSQNQFS EHDKT

Specificity: This antibody is expected to recognize both reported isoforms (NP_003248.3, NP_783297.2)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-PKPTSQNQFSEHDKT

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cellular junction of A431 cells, and in the plasma membrane and cytoplasm of U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: TJP1

Background: TJP1, tight junction protein 1 (zona occludens 1), DKFZp686M05161, MGC133289, ZO-1, OTTHUMP00000174520, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein

Gene ID: 7082

NCBI Accession: NP_003248, NP_783297

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization