PAR1 antibody (Internal Region)

Catalog No. ABIN570970

This Goat Polyclonal antibody specifically detects PAR1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PAR1 antibody (Internal Region) (ABIN570970)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PAR1 Antibody

Binding Specificity: Internal Region

Purpose: F2R / PAR1

Sequence: TRARRPESKA TNAT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TRARRPESKATNAT, from the Internal region (near N Terminus) of the protein sequence according to NP_001983.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell line HeLa (calculated MW of 47.4 kDa according to NP_001983.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R

Background: F2R, coagulation factor II (thrombin) receptor, CF2R, HTR, PAR1, TR, coagulation factor II receptor, protease-activated receptor 1, thrombin receptor

Gene ID: 2149

NCBI Accession: NP_001983

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus