Connexin 43/GJA1 antibody (Internal Region)

Catalog No. ABIN570972

This anti-Connexin 43/GJA1 antibody is a Goat Polyclonal antibody detecting Connexin 43/GJA1 in WB and ELISA. Suitable for Human and Rat.

Quick Overview for Connexin 43/GJA1 antibody (Internal Region) (ABIN570972)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: Internal Region

Purpose: Connexin 43 / GJA1

Sequence: QPFDFPDDNQ NSKK

Cross-Reactivity: Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPFDFPDDNQNSKK, from the internal region of the protein sequence according to NP_000156.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human and Rat Heart lysates (calculated MW of 43.0 kDa according to Human NP_000156.1 and Rat NP_036699.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1

Background: GJA1, gap junction protein, alpha 1, 43 kDa, CX43, DFNB38, GJAL, ODDD, connexin 43, gap junction 43 kDa heart protein, gap junction protein, alpha-like

Gene ID: 2697, 24392

NCBI Accession: NP_000156

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization