RAP1 antibody (C-Term)

Catalog No. ABIN570985

This Goat Polyclonal antibody specifically detects RAP1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for RAP1 antibody (C-Term) (ABIN570985)

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RAP1 Antibody

Binding Specificity: C-Term

Purpose: RAP1 / TERF2IP

Sequence: GAQNVARRIE FRKK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-GAQNVARRIEFRKK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Bone Marrow, Lymph Node and Tonsil lysates (calculated MW of 44.3 kDa according to NP_061848.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAP1

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Alternative Name: TERF2IP

Background: TERF2IP, telomeric repeat binding factor 2, interacting protein, DRIP5, RAP1, TRF2-interacting telomeric RAP1 protein, dopamine receptor interacting protein 5

Gene ID: 54386, 57321, 307861

NCBI Accession: NP_061848

Pathways: Cell Division Cycle, Telomere Maintenance