PPP2R4 antibody (C-Term)

Catalog No. ABIN570992

The Goat Polyclonal anti-PPP2R4 antibody has been validated for WB, IHC and ELISA. It is suitable to detect PPP2R4 in samples from Human, Mouse and Rat.

Quick Overview for PPP2R4 antibody (C-Term) (ABIN570992)

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PPP2R4 Antibody

Binding Specificity: C-Term

Purpose: PPP2R4 / PP2A

Sequence: GSLLPIHPVT SG

Specificity: This antibody is expected to recognize all reported isoforms (NP_821068.1, NP_066954.2, NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GSLLPIHPVTSG, from the C Terminus of the protein sequence according to NP_821068.1, NP_066954.2, NP_821070.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa band observed in Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 (calculated MW of 36.8 kDa according to NP_066954.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Alternative Name: PTPA

Background: PPP2R4, protein phosphatase 2A activator, regulatory subunit 4, MGC2184, PP2A, PR53, PTPA, OTTHUMP00000022333, PP2A phosphatase activator, PP2A, subunit B', phosphotyrosyl phosphatase activator, protein phosphatase 2A, regulatory subunit B', protein phosp

Gene ID: 5524, 110854, 362102

NCBI Accession: NP_821068, NP_066954, NP_821070

Pathways: PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades