CPNE1 antibody (C-Term)

Catalog No. ABIN570993

This anti-CPNE1 antibody is a Goat Polyclonal antibody detecting CPNE1 in WB and ELISA. Suitable for Human.

Quick Overview for CPNE1 antibody (C-Term) (ABIN570993)

Target: CPNE1 (Copine I (CPNE1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CPNE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CPNE1 Antibody

Binding Specificity: C-Term

Purpose: Copine 1

Sequence: APLKPLPPSA KDPA

Specificity: This antibody is expected to recognize isoform a and b (NP_003906.2, NP_690902.1). Reported variants represent identical protein: NP_690905.1, NP_690902.1, NP_690903.1, NP_690906.1, NP_690904.1, NP_690907.1, NP_690908.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-APLKPLPPSAKDPA

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Skin lysates (calculated MW of 59.1 kDa according to NP_690902.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CPNE1

Target: CPNE1 (Copine I (CPNE1))

Alternative Name: CPNE1

Background: CPNE1, copine I, COPN1, CPN1, MGC1142, OTTHUMP00000030775, OTTHUMP00000030776, copine-1

Gene ID: 8904

NCBI Accession: NP_690902, NP_003906