Decorin antibody (Internal Region)

Catalog No. ABIN571007

Product Details anti-Decorin Antibody

Target: Decorin (DCN)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Decorin

Sequence: KISRVDAASL KGLNN

Specificity: This antibody is expected to recognize the three reported isoforms a, b and c (NP_001911.1, NP_598011.1, NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.

Cross-Reactivity: Cow, Guinea Pig, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KISRVDAASLKGLNN, from the internal region of the protein sequence according to NP_001911.1, NP_598011.1, NP_598012.1,.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 38 kDa band observed in Human Kidney lysates (calculated MW of 39.7 kDa according to NP_001911.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN (DCN Products)

Synonyms: CSCD antibody, DSPG2 antibody, PG40 antibody, PGII antibody, PGS2 antibody, SLRR1B antibody, DC antibody, mDcn antibody, cscd antibody, dspg2 antibody, pg40 antibody, pgii antibody, pgs2 antibody, slrr1b antibody, decorin antibody, decorin L homeolog antibody, DCN antibody, Dcn antibody, dcn.L antibody, dcn antibody

Background: DCN, decorin, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, bone proteoglycan II, decorin proteoglycan, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B

Gene ID: 1634

NCBI Accession: NP_001911, NP_598011, NP_598012

Pathways: Glycosaminoglycan Metabolic Process