GGCX antibody (C-Term)

Catalog No. ABIN571011

This anti-GGCX antibody is a Goat Polyclonal antibody detecting GGCX in ELISA, WB and IHC. Suitable for Human.

Quick Overview for GGCX antibody (C-Term) (ABIN571011)

Target: GGCX (gamma-Glutamyl Carboxylase (GGCX))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GGCX antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GGCX Antibody

Binding Specificity: C-Term

Purpose: GGCX

Sequence: PPESNPDPVH SE

Specificity: This antibody is expected to recognize both isoforms (NP_000812.2, NP_001135741.1).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PPESNPDPVHSE, from the C Terminus of the protein sequence according to NP_000812.2, NP_001135741.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows distinct staining in the cytoplasm of epthelial cells. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 85 kDa band observed in Human Muscle lysates (calculated MW of 85 kDa according to NP_000812.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GGCX

Target: GGCX (gamma-Glutamyl Carboxylase (GGCX))

Alternative Name: GGCX

Background: GGCX, gamma-glutamyl carboxylase, FLJ26629, VKCFD1, vitamin K-dependent gamma-carboxylase

Gene ID: 2677

NCBI Accession: NP_000812, NP_001135741

Pathways: SARS-CoV-2 Protein Interactome