IREB2 antibody (Internal Region)

Catalog No. ABIN571014

This anti-IREB2 antibody is a Goat Polyclonal antibody detecting IREB2 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for IREB2 antibody (Internal Region) (ABIN571014)

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IREB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IREB2 Antibody

Binding Specificity: Internal Region

Purpose: IREB2 / IRP2

Sequence: SIHYEGSEYK LSHGS

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SIHYEGSEYKLSHGS

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6 μg/mL.

Western Blot: Approx 110 kDa band observed in Human Liver lysates (calculated MW of 105 kDa according to NP_004127.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This band was confir

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Alternative Name: IREB2

Background: IREB2, iron-responsive element binding protein 2, ACO3, FLJ23381, IRP2, IRP2AD, iron regulatory protein 2

Gene ID: 3658, 64602, 64831

NCBI Accession: NP_004127

Pathways: Transition Metal Ion Homeostasis