NUDC antibody (Internal Region)

Catalog No. ABIN571024

This Goat Polyclonal antibody specifically detects NUDC in WB, ELISA and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for NUDC antibody (Internal Region) (ABIN571024)

Target: NUDC

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NUDC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-NUDC Antibody

Binding Specificity: Internal Region

Purpose: NUDC

Sequence: PNLGNGADLP NYR

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PNLGNGADLPNYR, from the internal region of the protein sequence according to NP_006591.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38 kDa in lysates of cell line KNRK (calculated MW of 38.2 kDa according to Human NP_006591.1) and 38.4 kDa according to Rat NP_058967.1). Recommended concen

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NUDC

Target: NUDC

Background: NUDC, nuclear distribution gene C homolog (A. nidulans), HNUDC, MNUDC, NPD011, OTTHUMP00000004405, nuclear distribution gene C homolog

Gene ID: 10726, 18221, 29648

NCBI Accession: NP_006591