PRMT5 antibody (Internal Region)

Catalog No. ABIN571028

The Goat Polyclonal anti-PRMT5 antibody is suitable to detect PRMT5 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for PRMT5 antibody (Internal Region) (ABIN571028)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRMT5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PRMT5 Antibody

Binding Specificity: Internal Region

Purpose: PRMT5

Sequence: RACREKDRDP EAQ

Specificity: This antibody is expected to recognize both isoforms (NP_006100.2, NP_001034708.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: RACREKDRDPEAQ

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Jurkat lysates (calculated MW of 72.7 kDa and 71.3 kDa according to NP_006100.2, NP_001034708.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5

Background: PRMT5, protein arginine methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog

Gene ID: 10419, 27374, 364382

NCBI Accession: NP_006100, NP_001034708

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development