Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6) (C-Term) antibody

Details for Product No. ABIN571038
Antigen
  • ASK2
  • MAPKKK6
  • MEKK6
  • Ask2
  • mitogen-activated protein kinase kinase kinase 6
  • MAP3K6
  • Map3k6
Epitope
C-Term
20
10
9
8
7
4
3
2
2
1
Reactivity
Rat (Rattus)
68
39
8
2
2
Host
Goat
58
13
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
6
5
5
5
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
53
53
46
5
3
2
2
1
Options
Purpose MAP3K6
Immunogen Peptide with sequence C-RAILAQRAGSTP, from the C Terminus of the protein sequence according to NP_004663.3.
Blocking Peptide Blocking peptide for this product available: ABIN2816003
Sequence RAILAQRAGS TP
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name MAP3K6 (MAP3K6 Antibody Abstract)
Background MAP3K6, mitogen-activated protein kinase kinase kinase 6, ASK2, MAPKKK6, MGC125653, MGC20114, OTTHUMP00000005063, apoptosis signal-regulating kinase 2
Gene ID 9064
NCBI Accession NP_004663
Application Notes

DS WB Results: Approx 140 kDa band observed in Rat Heart lysates (calculated MW of 143 kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Did you look for something else?