Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD40 Ligand antibody (FITC)

This anti-CD40 Ligand antibody is a Mouse Monoclonal antibody detecting CD40 Ligand in FACS. Suitable for Human and Non-Human Primate.
Catalog No. ABIN5710572

Quick Overview for CD40 Ligand antibody (FITC) (ABIN5710572)

Target

See all CD40 Ligand (CD40LG) Antibodies
CD40 Ligand (CD40LG)

Reactivity

  • 185
  • 83
  • 42
  • 12
  • 10
  • 9
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Non-Human Primate

Host

  • 122
  • 99
  • 24
  • 11
  • 7
  • 2
  • 1
Mouse

Clonality

  • 149
  • 112
  • 4
Monoclonal

Conjugate

  • 148
  • 26
  • 25
  • 14
  • 7
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD40 Ligand antibody is conjugated to FITC

Application

  • 140
  • 130
  • 114
  • 74
  • 67
  • 54
  • 42
  • 21
  • 20
  • 15
  • 13
  • 13
  • 13
  • 6
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

24-31
  • Purpose

    Anti-Hu CD154 FITC

    Specificity

    The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

    Cross-Reactivity (Details)

    Human, Non-Human Primates

    Purification

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    human CD154 fusion protein

    Isotype

    IgG1
  • Application Notes

    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

    Restrictions

    For Research Use only
  • Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target

    CD40 Ligand (CD40LG)

    Alternative Name

    CD154

    Background

    CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

    Gene ID

    21947

    UniProt

    P27548

    Pathways

    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
You are here:
Chat with us!