GPR17 antibody (C-Term)

Catalog No. ABIN571066

This Goat Polyclonal antibody specifically detects GPR17 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for GPR17 antibody (C-Term) (ABIN571066)

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GPR17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-GPR17 Antibody

Binding Specificity: C-Term

Purpose: GPR17

Sequence: EGKTNESSLS AKSE

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EGKTNESSLSAKSE, from the C Terminus of the protein sequence according to NP_005282.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Mouse Brain lysates (calculated MW of 37.8 kDa according to Mouse NP_001020552.1 ). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa cells and additionally in the plasma membrane of U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GPR17

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Alternative Name: GPR17

Background: GPR17, G protein-coupled receptor 17, DKFZp686M18273

Gene ID: 2840, 574402, 767613

NCBI Accession: NP_005282