EYA1 antibody (Internal Region)

Catalog No. ABIN571076

The Goat Polyclonal anti-EYA1 antibody is suitable to detect EYA1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for EYA1 antibody (Internal Region) (ABIN571076)

Target: EYA1 (Eyes Absent Homolog 1 (EYA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EYA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-EYA1 Antibody

Binding Specificity: Internal Region

Purpose: EYA1

Sequence: TDPTAEYSTI HSP

Specificity: This antibody is expected to recognize all isoforms (NP_742057.1, NP_000494.2, NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TDPTAEYSTIHSP, from the internal region of the protein sequence according to NP_742057.1, NP_000494.2, NP_742056.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in lysates of cell line HEK293 (calculated MW of 60.7 kDa according to NP_742056.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EYA1

Target: EYA1 (Eyes Absent Homolog 1 (EYA1))

Alternative Name: EYA1

Background: EYA1, eyes absent homolog 1 (Drosophila), BOP, BOR, MGC141875, Eyes absent, Drosophila, homolog of, 1, Melnick-Fraser syndrome, OTTHUMP00000195053, eyes absent 1

Gene ID: 2138, 14048, 502935

NCBI Accession: NP_742057, NP_000494, NP_742056

Pathways: Sensory Perception of Sound, Positive Regulation of Response to DNA Damage Stimulus