GPI antibody (AA 81-93)

Catalog No. ABIN571080

This anti-GPI antibody is a Goat Polyclonal antibody detecting GPI in WB, ELISA and IHC. Suitable for Human.

Quick Overview for GPI antibody (AA 81-93) (ABIN571080)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GPI Antibody

Binding Specificity: AA 81-93

Purpose: GPI / Neuroleukin (aa81-93)

Sequence: RERMFNGEKI NYT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RERMFNGEKINYT, from the internal region (near N Terminus) of the protein sequence according to NP_000166.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Heart lysates and approx 55 kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1 kDa according to NP_000166.2). Recommended concentration: 0.1-0.3 μg/mL. Prim

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI

Background: GPI, glucose phosphate isomerase, AMF, GNPI, NLK, PGI, PHI, SA-36, autocrine motility factor, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, neuroleukin, oxoisomerase, phosphoglucose isomerase, phosphohexomutase,

Gene ID: 2821

NCBI Accession: NP_000166