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SRGAP2 antibody (Internal Region)

This Goat Polyclonal antibody specifically detects SRGAP2 in WB, ELISA and IF. It exhibits reactivity toward Human.
Catalog No. ABIN571090

Quick Overview for SRGAP2 antibody (Internal Region) (ABIN571090)

Target

See all SRGAP2 Antibodies
SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Reactivity

  • 16
  • 12
  • 9
Human

Host

  • 16
  • 3
Goat

Clonality

  • 16
  • 3
Polyclonal

Conjugate

  • 12
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SRGAP2 antibody is un-conjugated

Application

  • 9
  • 6
  • 4
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade

Verified
  • Binding Specificity

    • 3
    • 2
    • 1
    • 1
    Internal Region

    Purpose

    SRGAP2

    Sequence

    KQEDRQTPRS PDST

    Specificity

    This antibody is expected to recognize isoform a (NP_056141.2).

    Cross-Reactivity

    Cow, Dog, Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 124 kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentrat

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

    Alternative Name

    SRGAP2

    Background

    SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2

    Gene ID

    23380, 14270, 360840

    NCBI Accession

    NP_056141
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