SRGAP2 antibody (Internal Region)

Catalog No. ABIN571090

This Goat Polyclonal antibody specifically detects SRGAP2 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for SRGAP2 antibody (Internal Region) (ABIN571090)

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRGAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-SRGAP2 Antibody

Binding Specificity: Internal Region

Purpose: SRGAP2

Sequence: KQEDRQTPRS PDST

Specificity: This antibody is expected to recognize isoform a (NP_056141.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 124 kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SRGAP2

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Alternative Name: SRGAP2

Background: SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2

Gene ID: 23380, 14270, 360840

NCBI Accession: NP_056141