SRGAP2 antibody (Internal Region)

Catalog No. ABIN571090

Product Details anti-SRGAP2 Antibody

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRGAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: SRGAP2

Sequence: KQEDRQTPRS PDST

Specificity: This antibody is expected to recognize isoform a (NP_056141.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 124 kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SRGAP2

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Alternative Name: SRGAP2 (SRGAP2 Products)

Synonyms: ARHGAP34 antibody, FNBP2 antibody, SRGAP2A antibody, SRGAP3 antibody, 9930124L22Rik antibody, AI448945 antibody, FBP2 antibody, Fnbp2 antibody, srGAP3 antibody, AI452337 antibody, Arhgap14 antibody, D130026O08Rik antibody, Gbi antibody, MEGAP antibody, WRP antibody, mKIAA0411 antibody, RGD1566016 antibody, srGAP2 antibody, srgap2a antibody, wu:fb47h12 antibody, zgc:136814 antibody, fnbp2 antibody, srgap2 antibody, SLIT-ROBO Rho GTPase activating protein 2 antibody, SLIT-ROBO Rho GTPase activating protein 3 antibody, SLIT-ROBO Rho GTPase activating protein 2 L homeolog antibody, SRGAP2 antibody, Srgap2 antibody, Srgap3 antibody, srgap2 antibody, srgap2.L antibody

Background: SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2

Gene ID: 23380, 14270, 360840

NCBI Accession: NP_056141