Mesothelin antibody (Internal Region)

Catalog No. ABIN571102

This Goat Polyclonal antibody specifically detects Mesothelin in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for Mesothelin antibody (Internal Region) (ABIN571102)

Target: Mesothelin (MSLN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-Mesothelin Antibody

Binding Specificity: Internal Region

Purpose: Mesothelin

Sequence: CPSGKKAREI DES

Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_005814.2, NP_037536.2).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CPSGKKAREIDES, from the internal region of the protein sequence according to NP_005814.2, NP_037536.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Placenta lysates (calculated MW of 68.1 kDa according to NP_005814.2 and of 69.0 kDa according to NP_037536.2). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consist

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background: MSLN, mesothelin, CAK1, MPF, SMR, megakaryocyte potentiating factor

Gene ID: 10232

NCBI Accession: NP_005814, NP_037536

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process