ACO2 antibody (Internal Region)

Catalog No. ABIN571124

The Goat Polyclonal anti-ACO2 antibody is suitable to detect ACO2 in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ACO2 antibody (Internal Region) (ABIN571124)

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ACO2 Antibody

Binding Specificity: Internal Region

Purpose: Aconitase 2

Sequence: QHVDVSPTSQ RLQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QHVDVSPTSQRLQ, from the internal region of the protein sequence according to NP_001089.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 85.4 kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Customer finds weak pixulate staining in the cytoplasm of HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2

Background: ACO2, aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908, aconitate hydratase, citrate hydro-lyase

Gene ID: 50, 11429, 79250

NCBI Accession: NP_001089