ACO2 antibody (AA 541-555)

Catalog No. ABIN571125

The Goat Polyclonal anti-ACO2 antibody (ABIN571125) specifically detects ACO2 in WB, ELISA and IHC. The antibody is reactive with Human, Rat, Mouse and Pig samples.

Quick Overview for ACO2 antibody (AA 541-555) (ABIN571125)

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ACO2 Antibody

Binding Specificity: AA 541-555

Purpose: Aconitase 2 (aa541-555)

Sequence: QDTYQHPPKD SSGQH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDTYQHPPKDSSGQH, from the internal region of the protein sequence according to NP_001089.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4 kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2

Background: ACO2, aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908, aconitate hydratase, citrate hydro-lyase

Gene ID: 50, 11429, 79250

NCBI Accession: NP_001089