DCUN1D1 antibody (C-Term)

Catalog No. ABIN571140

The Goat Polyclonal anti-DCUN1D1 antibody (ABIN571140) specifically detects DCUN1D1 in ELISA, WB and IHC. The antibody is reactive with Human samples.

Quick Overview for DCUN1D1 antibody (C-Term) (ABIN571140)

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DCUN1D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-DCUN1D1 Antibody

Binding Specificity: C-Term

Purpose: DCUN1D1

Sequence: RPQIAGTKST T

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPQIAGTKSTT, from the C Terminus of the protein sequence according to NP_065691.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 28 kDa band observed in HepG2 lysates (calculated MW of 30.1 kDa according to NP_065691.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DCUN1D1

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Alternative Name: DCUN1D1

Background: DCUN1D1, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae), DCUN1L1, RP42, SCCRO, SCRO, Tes3, RP42 homolog, squamous cell carcinoma-related oncogene

Gene ID: 54165, 114893

NCBI Accession: NP_065691