ALDH2 antibody (Internal Region)

Catalog No. ABIN571181

The Goat Polyclonal anti-ALDH2 antibody has been validated for WB and ELISA. It is suitable to detect ALDH2 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for ALDH2 antibody (Internal Region) (ABIN571181)

Target: ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ALDH2 Antibody

Binding Specificity: Internal Region

Purpose: ALDH2

Sequence: DETQFKKILG YIN

Specificity: This antibody is expected to recognize both reported isoforms (NP_000681.2, NP_001191818.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DETQFKKILGYIN, from the internal region of the protein sequence according to NP_000681.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 56.4 kDa according to Human NP_000681.2 amd 56.5 kDa according to Mouse NP_033786.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ALDH2 antibody (ABIN571181)

Gergs, Jahn, Schulz, Großmann, Rueckschloss, Demus, Buchwalow, Neumann: "Protein Phosphatase 2A Improves Cardiac Functional Response to Ischemia and Sepsis." in: International journal of molecular sciences, Vol. 23, Issue 9, (2022) (PubMed).

Target Details for ALDH2

Target: ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))

Alternative Name: ALDH2

Background: ALDH2, aldehyde dehydrogenase 2 family (mitochondrial), ALDH-E2, ALDHI, ALDM, MGC1806, ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH, mitochondrial aldehyde dehydrogenase 2, nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Gene ID: 217, 11669, 29539

NCBI Accession: NP_000681, NP_001191818