ETFA antibody (AA 139-152)

Catalog No. ABIN571194

This anti-ETFA antibody is a Goat Polyclonal antibody detecting ETFA in WB, IHC and ELISA. Suitable for Human.

Quick Overview for ETFA antibody (AA 139-152) (ABIN571194)

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ETFA Antibody

Binding Specificity: AA 139-152

Purpose: ETFA (aa139-152)

Sequence: KSPDTFVRTI YAGN

Specificity: This antibody is expected to recognize both isoforms (NP_000117.1, NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KSPDTFVRTIYAGN

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland and Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30+35 kDa bands observed in Human Colon lysates (calculated MW of 35.1 kDa according to NP_000117.1 and of 30.0 kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ETFA

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Alternative Name: ETFA

Background: ETFA, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, MADD, electron transfer flavoprotein alpha-subunit, electron transfer flavoprotein, alpha polypeptide, glutaric aciduria II

Gene ID: 2108, 110842, 300726

NCBI Accession: NP_000117, NP_001121188

Pathways: SARS-CoV-2 Protein Interactome