UCHL1 antibody (AA 211-221)

Catalog No. ABIN571207

This Goat Polyclonal antibody specifically detects UCHL1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for UCHL1 antibody (AA 211-221) (ABIN571207)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-UCHL1 Antibody

Binding Specificity: AA 211-221

Purpose: UCHL1 (aa 211-221)

Sequence: EVRFSAVALC K

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: EVRFSAVALCK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Brain (Hippocampus) and Mouse and Rat Brain lysates (calculated MW of 24.8 kDa according to Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1

Background: UCHL1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), PARK5, PGP9.5, PGP95, Uch-L1, neuron cytoplasmic protein 9.5, ubiquitin C-terminal hydrolase, ubiquitin carboxyl-terminal esterase L1

Gene ID: 7345, 22223, 29545

NCBI Accession: NP_004172

Pathways: Feeding Behaviour