Phospholamban antibody (N-Term)

Catalog No. ABIN571208

The Goat Polyclonal anti-Phospholamban antibody is suitable to detect Phospholamban in samples from Human and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for Phospholamban antibody (N-Term) (ABIN571208)

Target: Phospholamban (PLN)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Phospholamban Antibody

Binding Specificity: N-Term

Purpose: Phospholamban / PLN

Sequence: KVQYLTRSAI RR

Cross-Reactivity: Cow, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: KVQYLTRSAIRR-C

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 13 kDa band observed in Human and Rat Heart lysates (calculated MW of 6.11 kDa according to NP_002658.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLN

Background: PLN, phospholamban, CMD1P, PLB, cardiac phospholamban

Gene ID: 5350, 64672

NCBI Accession: NP_002658

Pathways: Negative Regulation of Transporter Activity