MYO5A antibody (Internal Region)

Catalog No. ABIN571223

The Goat Polyclonal anti-MYO5A antibody has been validated for WB, ELISA and IF. It is suitable to detect MYO5A in samples from Human and Mouse.

Quick Overview for MYO5A antibody (Internal Region) (ABIN571223)

Target: MYO5A (Myosin VA (MYO5A))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MYO5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-MYO5A Antibody

Binding Specificity: Internal Region

Purpose: MYO5A

Sequence: ETKQLELDLN

Specificity: This antibody is expected to recognize both reported isoforms (NP_000250.3, NP_001135967.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ETKQLELDLN

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 200 kDa band observed in lysates of cell line Jurkat (calculated MW of 215 kDa according to NP_000250.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, P

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Alternative Name: MYO5A

Background: MYO5A, myosin VA (heavy chain 12, myoxin), GS1, MYH12, MYO5, MYR12, dilute, myosin V, myosin VA, myosin, heavy polypeptide kinase, myoxin

Gene ID: 4644, 17918, 25017

NCBI Accession: NP_000250, NP_001135967

Pathways: Hormone Transport, Peptide Hormone Metabolism