PSMB3 antibody (Internal Region)

Catalog No. ABIN571231

This Goat Polyclonal antibody specifically detects PSMB3 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PSMB3 antibody (Internal Region) (ABIN571231)

Target: PSMB3 (Proteasome Subunit beta 3 (PSMB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PSMB3 Antibody

Binding Specificity: Internal Region

Purpose: PSMB3

Sequence: NLYELKEGRQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NLYELKEGRQ

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 10 μg/mL.

Western Blot: Approx 24 kDa band observed in lysates of cell line K562 (calculated MW of 22.9 kDa according to NP_002786.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PSMB3

Target: PSMB3 (Proteasome Subunit beta 3 (PSMB3))

Alternative Name: PSMB3

Background: PSMB3, proteasome (prosome, macropain) subunit, beta type, 3, HC10-II, MGC4147, proteasome beta 3 subunit, proteasome chain 13, proteasome component C10-II, proteasome theta chain

Gene ID: 5691, 26446, 29676

NCBI Accession: NP_002786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Cell RedoxHomeostasis, Lipid Metabolism