POR antibody (Internal Region)

Catalog No. ABIN571235

The Goat Polyclonal anti-POR antibody has been validated for WB, IHC and ELISA. It is suitable to detect POR in samples from Human, Rat and Mouse.

Quick Overview for POR antibody (Internal Region) (ABIN571235)

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-POR Antibody

Binding Specificity: Internal Region

Purpose: CYPOR

Sequence: TNPPRTNVLY E

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TNPPRTNVLYE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 90 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 77.0 kDa according to NP_000932.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Alternative Name: POR

Background: POR, P450 (cytochrome) oxidoreductase, CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R, NADPH-dependent cytochrome P450 reductase, cytochrome P450 reductase

Gene ID: 5447, 18984, 29441

NCBI Accession: NP_000932

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome