PTCHD1 antibody (Patched Domain Containing 1) (Internal Region)

Details for Product anti-PTCHD1 Antibody No. ABIN571261
Antigen
Epitope
Internal Region
13
9
8
7
1
Reactivity
Human
17
15
Host
Goat
17
6
Clonality
Polyclonal
Conjugate
This PTCHD1 antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
23
20
15
2
1
Options
Purpose PTCHD1
Immunogen C-DFQKTSRVSER
Blocking Peptide Blocking peptide for this product available: ABIN2816342
Sequence DFQKTSRVSE R
Isotype IgG
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name PTCHD1 (PTCHD1 Antibody Abstract)
Background PTCHD1, patched domain containing 1, FLJ30296, MGC149798
Gene ID 139411
NCBI Accession NP_775766
Application Notes

DS WB Results: Approx 100 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 101 kDa according to NP_775766.2). Recommended concentration: 0.5-2 μg/mL. An additional band of unknown identity was also consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Patched Domain Containing 1 (PTCHD1) (Internal Region) antibody (ABIN571261) ABIN571261 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffe...