NEK7 antibody (Internal Region)

Catalog No. ABIN571271

The Goat Polyclonal anti-NEK7 antibody has been validated for WB, ELISA and IHC. It is suitable to detect NEK7 in samples from Human.

Quick Overview for NEK7 antibody (Internal Region) (ABIN571271)

Target: NEK7

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NEK7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NEK7 Antibody

Binding Specificity: Internal Region

Purpose: NEK7

Sequence: NTLANFRIEK K

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NTLANFRIEKK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_598001.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NEK7

Target: NEK7

Alternative Name: NEK7

Background: NEK7, NIMA (never in mitosis gene a)-related kinase 7, NIMA-related kinase 7

Gene ID: 140609, 59125, 360850

NCBI Accession: NP_598001

Pathways: Inflammasome