EIF3E antibody (C-Term)

Catalog No. ABIN571273

This Goat Polyclonal antibody specifically detects EIF3E in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for EIF3E antibody (C-Term) (ABIN571273)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-EIF3E Antibody

Binding Specificity: C-Term

Purpose: EIF3E

Sequence: KLNQNSRSEA PN

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KLNQNSRSEAPN

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in lysates of cell line Daudi (calculated MW of 52.2 kDa according to NP_001559.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background: EIF3E, eukaryotic translation initiation factor 3, subunit E, EIF3-P48, EIF3S6, INT6, eIF3-p46, eukaryotic translation initiation factor 3, subunit 6 (48kD), eukaryotic translation initiation factor 3, subunit 6 48 kDa, mammary tumor-associated protein INT

Gene ID: 3646

NCBI Accession: NP_001559

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C