IDH2 antibody (Internal Region)

Catalog No. ABIN571275

The Goat Polyclonal anti-IDH2 antibody (ABIN571275) specifically detects IDH2 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Rat and Pig samples.

Quick Overview for IDH2 antibody (Internal Region) (ABIN571275)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IDH2 Antibody

Binding Specificity: Internal Region

Purpose: IDH2

Sequence: CIHGLSNVKL NE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CIHGLSNVKLNE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 48 kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9 kDa according to Mouse NP_766599.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2

Background: IDH2, isocitrate dehydrogenase 2 (NADP+), mitochondrial, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH, NADP+-specific ICDH, isocitrate dehydrogenase, mitochondrial, oxalosuccinate decarboxylase

Gene ID: 3418, 269951, 361596

NCBI Accession: NP_002159

Pathways: Warburg Effect