IDH2 antibody (Internal Region)

Catalog No. ABIN571275

This anti-IDH2 antibody is a Goat Polyclonal antibody detecting IDH2 in WB, IHC and ELISA. Suitable for Human, Mouse, Rat and Pig.

Quick Overview for IDH2 antibody (Internal Region) (ABIN571275)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IDH2 Antibody

Binding Specificity: Internal Region

Purpose: IDH2

Sequence: CIHGLSNVKL NE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CIHGLSNVKLNE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 48 kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9 kDa according to Mouse NP_766599.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2

Background: IDH2, isocitrate dehydrogenase 2 (NADP+), mitochondrial, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH, NADP+-specific ICDH, isocitrate dehydrogenase, mitochondrial, oxalosuccinate decarboxylase

Gene ID: 3418, 269951, 361596

NCBI Accession: NP_002159

Pathways: Warburg Effect