SIM2 antibody (Internal Region)

Catalog No. ABIN571281

This anti-SIM2 antibody is a Goat Polyclonal antibody detecting SIM2 in ELISA and WB. Suitable for Mouse.

Quick Overview for SIM2 antibody (Internal Region) (ABIN571281)

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIM2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-SIM2 Antibody

Binding Specificity: Internal Region

Purpose: SIM2

Sequence: SDLLYTPSYS

Specificity: This antibody is expected to recognize all reported isoforms (NP_005060.1, NP_033664.2).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SDLLYTPSYS

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in fetal Mouse Kidney lysates (calculated MW of 73.2 kDa according to Human NP_005060.1) and 72.5 kDa according to Mouse NP_035507.2. Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIM2

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Alternative Name: SIM2

Background: SIM2, single-minded homolog 2 (Drosophila), MGC119447, SIM, bHLHe15, OTTHUMP00000109044, class E basic helix-loop-helix protein 15, single-minded homolog 2, single-minded homolog 2, short isoform, transcription factor SIM2

Gene ID: 6493, 20465, 304071

NCBI Accession: NP_005060, NP_033664