E2F1 antibody (Internal Region, Lys120)

Catalog No. ABIN571306

This anti-E2F1 antibody is a Rabbit Polyclonal antibody detecting E2F1 in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for E2F1 antibody (Internal Region, Lys120) (ABIN571306)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-E2F1 Antibody

Binding Specificity: Internal Region, Lys120

Specificity: Reacts with human E2F-1 acK120 and acK125

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): May cross-react with E2F-1 from mouse and other species due to sequence homology

Purification: Antiserum

Immunogen: Synthetic peptide derived from internal domain of human E2F-1 protein containing acetyl lysine 120 and 125

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilutions should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: Transcription Factor E2F1 (E2F-1)

Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy