NG2 antibody (Extracellular Domain)

Catalog No. ABIN571935

This anti-NG2 antibody is a Rabbit Polyclonal antibody detecting NG2 in WB and IHC. Suitable for Mouse.

Quick Overview for NG2 antibody (Extracellular Domain) (ABIN571935)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NG2 Antibody

Binding Specificity: Extracellular Domain

Purpose: Rabbit antibody to NG2

Specificity: Specific for NG2.

Cross-Reactivity: Mouse

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: NG2

Background: Valuable marker for several incompletely differentiated precursor cells. Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4 beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42 ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Subcellular location: Apical cell membrane, Single-pass type I membrane protein, Extracellular side. Cell projection ‰ lamellipodium membrane, Single-pass type I membrane protein, Extracellular side. Tissue specificity: Expressed in microcascular pericytes and not endothelial cells.

UniProt: Q8VHY0

Pathways: Glycosaminoglycan Metabolic Process